WB of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by WB against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ul of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by WB. In this blot the antibody was used at a 1:10,000 dilution.