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ProductsBack/FGFR2 / FGF Receptor 2 Antibody (clone OTI5C5)

FGFR2 / FGF Receptor 2 Antibody (clone OTI5C5)

LS-C173091
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFGFR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FGFR2 / FGF Receptor 2 Antibody (clone OTI5C5)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:100), IHC, IHC-P (1:150), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5C5
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2263
  • Target name
    FGFR2
  • Target description
    fibroblast growth factor receptor 2
  • Target synonyms
    bacteria-expressed kinase; BBDS; BEK; BEK fibroblast growth factor receptor; BFR-1; CD332; CEK3; CFD1; ECT1; fibroblast growth factor receptor 2; JWS; keratinocyte growth factor receptor; KGFR; K-SAM; protein tyrosine kinase, receptor like 14; TK14; TK25
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGFR2 using anti-FGFR2 antibody (A00231-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 antigen affinity purified polyclonal antibody (Catalog # A00231-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR2 at approximately 145 kDa. The expected band size for FGFR2 is at 92 kDa.
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