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FIS1 antibody

GTX111010
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFIS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FIS1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51024
  • Target name
    FIS1
  • Target description
    fission, mitochondrial 1
  • Target synonyms
    CGI-135, TTC11, mitochondrial fission 1 protein, FIS1 homolog, H_NH0132A01.6, TPR repeat protein 11, fission 1 (mitochondrial outer membrane) homolog, hFis1, mitochondrial fission molecule, tetratricopeptide repeat domain 11, tetratricopeptide repeat protein 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3D6
  • Protein Name
    Mitochondrial fission 1 protein
  • Scientific Description
    The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Rostamzadeh F, Najafipour H, Aminizadeh S, et al. Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy. Biomed Pharmacother. 2024,170:116020. doi: 10.1016/j.biopha.2023.116020
    Read this paper
  • Su WP, Li CJ, Lin LT, et al. Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition. Biomed Pharmacother. 2023,163:114888. doi: 10.1016/j.biopha.2023.114888
    Read this paper
  • Arjona SP, Allen CNS, Santerre M, et al. Disruption of Mitochondrial-associated ER membranes by HIV-1 tat protein contributes to premature brain aging. CNS Neurosci Ther. 2023,29(1):365-377. doi: 10.1111/cns.14011
    Read this paper
  • Chen Z, Liang W, Hu J, et al. Sirt6 deficiency contributes to mitochondrial fission and oxidative damage in podocytes via ROCK1-Drp1 signalling pathway. Cell Prolif. 2022,55(10):e13296. doi: 10.1111/cpr.13296
    Read this paper
  • Li CJ, Lin LT, Tsai HW, et al. Phosphoglycerate mutase family member 5 maintains oocyte quality via mitochondrial dynamic rearrangement during aging. Aging Cell. 2022,21(2):e13546. doi: 10.1111/acel.13546
    Read this paper
  • Liu W, Fan Y, Ding H, et al. Normothermic machine perfusion attenuates hepatic ischaemia-reperfusion injury by inhibiting CIRP-mediated oxidative stress and mitochondrial fission. J Cell Mol Med. 2021,25(24):11310-11321. doi: 10.1111/jcmm.17062
    Read this paper
  • Zhu Z, Liang W, Chen Z, et al. Mitoquinone Protects Podocytes from Angiotensin II-Induced Mitochondrial Dysfunction and Injury via the Keap1-Nrf2 Signaling Pathway. Oxid Med Cell Longev. 2021,2021:1394486. doi: 10.1155/2021/1394486
    Read this paper
  • Lin TK, Lin KJ, Lin HY, et al. Glucagon-Like Peptide-1 Receptor Agonist Ameliorates 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) Neurotoxicity Through Enhancing Mitophagy Flux and Reducing α-Synuclein and Oxidative Stress. Front Mol Neurosci. 2021,14:697440. doi: 10.3389/fnmol.2021.697440
    Read this paper
  • Ma W, He S, Xu Y, et al. Ameliorative Effect of Sodium Selenite on Silver Nanoparticles-Induced Myocardiocyte Structural Alterations in Rats. Int J Nanomedicine. 2020,15:8281-8292. doi: 10.2147/IJN.S271457
    Read this paper
  • Lam HYP, Chen CC, Chen TT, et al. Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain. Parasitol Int. 2021,80:102231. doi: 10.1016/j.parint.2020.102231
    Read this paper

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Figure 1. Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: human HEK293 whole cell lysates, Lane 10: human A549 whole cell lysates, Lane 11: human Hela whole cell lysates, Lane 12: human Raji whole cell lysates, Lane 13: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
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