Western Blot Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Flag Tag Monoclonal Antibody
CSB-MA000021M0M
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityAll Species
Overview
- SupplierCusabio
- Product NameFlag Tag Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4G5C8
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionFlag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
- ReactivityAll Species
- Storage Instruction-20°C or -80°C
- UNSPSC12352203