Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Polyclonal Antibody at dilution of 1:1000.
Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Polyclonal Antibody at dilution of 1:1000.
Flag-Tag Polyclonal Antibody
E-AB-20045
Product group Antibodies
Overview
- SupplierElabscience
- Product NameFlag-Tag Polyclonal Antibody
- Delivery Days Customer12
- CertificationResearch Use Only
- Scientific DescriptionProtein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
- UNSPSC12352203