Western Blot of Biotin Conjugated Goat Anti-Fluorescein Antibody. Lane 1: BSA conjugated Fluorescein. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.
Western Blot of Biotin Conjugated Goat Anti-Fluorescein Antibody. Lane 1: BSA conjugated Fluorescein. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.
Fluorescein antibody (Biotin)
GTX26655
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameFluorescein antibody (Biotin)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000-1:10000. ELISA: 1:10000-1:50000. IHC: 1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateBiotin
- HostGoat
- IsotypeIgG
- Scientific DescriptionAffinity purified polyclonal anti-fluorescein can be used to detect both fluoresceinated proteins and nucleic acids.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Proteoglycan 4 Reduces Neuroinflammation and Protects the Blood-Brain Barrier after Traumatic Brain Injury. Bennett M et al., 2021 Feb 15, J NeurotraumaRead more