Fluorescein isothiocyanate isomer 1 antibody (HRP)

Biorbyt

Fluorescein isothiocyanate isomer 1 antibody (HRP)

10

Research Use Only

Monoclonal

NI 239

HRP

Mouse

IgG1

Horseradish peroxidase conjugated purified monoclonal mouse antibody to fluorescein isothiocyanate isomer 1. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin- avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only.

-20°C,2°C to 8°C

12352203