Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate All lanes: FOXA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate All lanes: FOXA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46 kDa Observed band size: 50 kDa
FOXA1 Recombinant Monoclonal Antibody
CSB-RA246102A0HU
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXA1
Overview
- SupplierCusabio
- Product NameFOXA1 Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3B2
- ConjugateUnconjugated
- Gene ID3169
- Target nameFOXA1
- Target descriptionforkhead box A1
- Target synonymsforkhead box protein A1; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF-3-alpha; TCF3A; TCF-3A; transcription factor 3A
- IsotypeIgG
- Protein IDP55317
- Protein NameHepatocyte nuclear factor 3-alpha
- Scientific DescriptionTranscription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a pioneer factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3 (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at Lys-5 (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203