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Galectin3 antibody detects Galectin3 protein by western blot analysis. A. 50 μg rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody (GTX125897) dilution: 1:1000
Galectin3 antibody detects Galectin3 protein by western blot analysis. A. 50 μg rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody (GTX125897) dilution: 1:1000
Galectin3 antibody detects Galectin3 protein by western blot analysis. A. 50 μg rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody (GTX125897) dilution: 1:1000

Galectin 3 antibody

GTX125897
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLGALS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Galectin 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3958
  • Target name
    LGALS3
  • Target description
    galectin 3
  • Target synonyms
    CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, galectin-3, 35 kDa lectin, IgE-binding protein, MAC-2 antigen, advanced glycation end-product receptor 3, carbohydrate-binding protein 35, epididymis secretory sperm binding protein, galactose-specific lectin 3, laminin-binding protein, lectin L-29, lectin, galactoside-binding, soluble, 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17931
  • Protein Name
    Galectin-3
  • Scientific Description
    This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sbroscia M, Di Gioacchino M, Ascenzi P, et al. Thyroid cancer diagnosis by Raman spectroscopy. Sci Rep. 2020,10(1):13342. doi: 10.1038/s41598-020-70165-0
    Read this paper
  • Li FY, Weng IC, Lin CH, et al. Helicobacter pylori induces intracellular galectin-8 aggregation around damaged lysosomes within gastric epithelial cells in a host O-glycan-dependent manner. Glycobiology. 2019,29(2):151-162. doi: 10.1093/glycob/cwy095
    Read this paper
  • Lin GL, Ting HJ, Tseng TC, et al. Modulation of the mRNA-binding protein HuR as a novel reversal mechanism of epirubicin-triggered multidrug resistance in colorectal cancer cells. PLoS One. 2017,12(10):e0185625. doi: 10.1371/journal.pone.0185625
    Read this paper
  • Brandi J, Dalla Pozza E, Dando I, et al. Secretome protein signature of human pancreatic cancer stem-like cells. J Proteomics. 2016,136:1-12. doi: 10.1016/j.jprot.2016.01.017
    Read this paper
  • Huang HJ, Lin CC, Chou HC, et al. Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells. Mol Biosyst. 2014,10(12):3086-100. doi: 10.1039/c4mb00451e
    Read this paper
  • Chemaly ER, Kang S, Zhang S, et al. Differential patterns of replacement and reactive fibrosis in pressure and volume overload are related to the propensity for ischaemia and involve resistin. J Physiol. 2013,591(21):5337-55. doi: 10.1113/jphysiol.2013.258731
    Read this paper
  • Chen JY, Chou HC, Chen YH, et al. High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease. J Nutr Biochem. 2013,24(11):1889-910. doi: 10.1016/j.jnutbio.2013.05.006
    Read this paper

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Figure 1. Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody (A00621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # A00621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.
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