GATA6 Antibody (C-Terminus) LS-B3483

Biorbyt

GATA6 Antibody (C-Terminus) LS-B3483

10

Not recommended for: IF. Further information: Immunohistochemistry: LS-B3483 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3483 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml) IHC, IHC-P, WB

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

Rabbit

IgG

GATA6 Antibody (C-Terminus) LS-B3483

Human

-20°C,2°C to 8°C

12352203