Western blot of Biotin Goat Anti-GFP antibody. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.
Western blot of Biotin Goat Anti-GFP antibody. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.
GFP antibody (Biotin)
GTX26658
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameGFP antibody (Biotin)
- Delivery Days Customer9
- Antibody SpecificityNo reaction was observed against Human, Mouse and Rat Serum Proteins.
- Application Supplier NoteWB: 1:2000-1:10000. IHC-P: 1:1000-1:5000. ELISA: 1:50000-1:80000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateBiotin
- HostGoat
- IsotypeIgG
- Scientific DescriptionDesigned to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling. Schmitt M et al., 2018 Aug 28, Cell RepRead more
- A novel role for C-C motif chemokine receptor 2 during infection with hypervirulent Mycobacterium tuberculosis. Dunlap MD et al., 2018 Nov, Mucosal ImmunolRead more
- Acinar cells in the neonatal pancreas grow by self-duplication and not by neogenesis from duct cells. Houbracken I et al., 2017 Oct 3, Sci RepRead more
- Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease. Manko A et al., 2017, PLoS OneRead more
- Isotachophoresis-Based Surface Immunoassay. Paratore F et al., 2017 Jul 18, Anal ChemRead more
- Tricellular Tight Junction Protein Tricellulin Is Targeted by the Enteropathogenic Escherichia coli Effector EspG1, Leading to Epithelial Barrier Disruption. Morampudi V et al., 2017 Jan, Infect ImmunRead more
- Beta Cell Mass Restoration in Alloxan-Diabetic Mice Treated with EGF and Gastrin. Song I et al., 2015, PLoS OneRead more