1:1000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1microg (2) and 10microg (3) of cell lysates used
1:1000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1microg (2) and 10microg (3) of cell lysates used
GFP antibody [GF28R]
GTX82564
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameGFP antibody [GF28R]
- Delivery Days Customer9
- Antibody SpecificityRecognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
- Application Supplier NoteRecommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-3000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGF28R
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionA highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203