Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20oC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20oC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
GFP antibody (HRP)
GTX26663
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameGFP antibody (HRP)
- Delivery Days Customer9
- Antibody SpecificityNo reaction was observed against Human, Mouse and Rat Serum Proteins.
- Application Supplier NoteWB: 1:2000-1:5000. ELISA: 1:10000-1:50000. IHC: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateHRP
- HostGoat
- IsotypeIgG
- Scientific DescriptionDesigned to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation. Leen EN et al., 2016 Jan, PLoS PathogRead more