WB using Anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells overexpressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1, 350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L].