Gli-2 antibody

Biorbyt

Gli-2 antibody

16

This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Western Blot, ELISA, ImmunoHistoChemistry

ELISA: 1:2,000 - 1:12,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000 ELISA, IHC, WB

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

Liquid

GLI2

GLI family zinc finger 2

CJS; GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9; oncogene GLI2; PHS2; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP1; THP2; zinc finger protein GLI2

Rabbit

IgG

Zinc finger protein GLI2

Gli-2 antibody

Human, Rat

-20°C,See manual

12352203