Genetex Goat anti Glutamine Synthetase antibody (GTX26585) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3,000 dilution of primary antibody (on 4 degree C).
Genetex Goat anti Glutamine Synthetase antibody (GTX26585) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3,000 dilution of primary antibody (on 4 degree C).
Glutamine Synthetase antibody
GTX26585
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityBacteria
Overview
- SupplierGeneTex
- Product NameGlutamine Synthetase antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:5000. IP: 1:100. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration90 mg/ml
- ConjugateUnconjugated
- HostGoat
- IsotypeIgG
- Scientific DescriptionGlutamine Synthetase (GS) catalyzes the conversion of ammonia and glutamate to glutamine. This reaction consumes a molecule of ATP:Glutamate + NH4+ + ATP --> Glutamine + ADP + Pi. GS is found in astrocytes as an octamer of identical 45kDa subunits. The function of GS is the detoxification of brain ammonia. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of GS in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of GS is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer s disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. On the other hands, the level of expression of GS is increased during ischemia in vivo or hypoxia in culture.
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203