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ProductsBack/Glycogen synthase 1 (phospho Ser641) antibody
Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 microM (lanes 2, 3 and 4 respectively) for 15 or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amershams substrate system.
Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 microM (lanes 2, 3 and 4 respectively) for 15 or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amershams substrate system.
Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 microM (lanes 2, 3 and 4 respectively) for 15 or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amershams substrate system.

Glycogen synthase 1 (phospho Ser641) antibody

GTX22479
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGYS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Glycogen synthase 1 (phospho Ser641) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:1000-1:5000. ELISA: 1:20000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2997
  • Target name
    GYS1
  • Target description
    glycogen synthase 1
  • Target synonyms
    GSY, GYS, glycogen [starch] synthase, muscle, glycogen synthase 1 (muscle)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13807
  • Protein Name
    Glycogen [starch] synthase, muscle
  • Scientific Description
    Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at Ser 640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Brull A, de Luna N, Blanco-Grau A, et al. Phenotype consequences of myophosphorylase dysfunction: insights from the McArdle mouse model. J Physiol. 2015,593(12):2693-706. doi: 10.1113/JP270085
    Read this paper

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