Goat Ig Against Human IgE Immunofixation

Biorbyt

Goat Ig Against Human IgE Immunofixation

10

Dot Blot, ImmunoElectrophoresis

DOT, IEP

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

Goat

IgG

Ig fraction of polyclonal goat antiserum to human immunoglobulin IgE, Fc specific. This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Highly purified monoclonal IgE isolated from human serum. Freunds complete adjuvant is used in the first step of the immunization procedure.

Human

12352203