Immunoprecipitation of Rock-2 protein from HeLa whole cell extracts using 5 microg goat IgG isotype control (GTX35039) or Rock-2 Goat pAb. Western blot analysis was performed using Rock-2 goat pAb. Goat IgG HRP (GTX228416-01) was used as a secondary reagent.
Immunoprecipitation of Rock-2 protein from HeLa whole cell extracts using 5 microg goat IgG isotype control (GTX35039) or Rock-2 Goat pAb. Western blot analysis was performed using Rock-2 goat pAb. Goat IgG HRP (GTX228416-01) was used as a secondary reagent.
Goat IgG isotype control
GTX35039
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
Overview
- SupplierGeneTex
- Product NameGoat IgG isotype control
- Delivery Days Customer9
- Application Supplier NoteUse at a concentration/dilution equivalent to that of your experimental primary antibody.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostGoat
- IsotypeIgG
- Scientific DescriptionInclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific background binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
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