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ProductsBack/GOLM1 / GP73 / GOLPH2 Antibody (clone OTI3D7)

GOLM1 / GP73 / GOLPH2 Antibody (clone OTI3D7)

LS-C173619
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGOLM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GOLM1 / GP73 / GOLPH2 Antibody (clone OTI3D7)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3D7
  • Concentration
    0.68 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51280
  • Target name
    GOLM1
  • Target description
    golgi membrane protein 1
  • Target synonyms
    bA379P1.3; C9orf155; epididymis luminal protein 46; Golgi membrane protein 1; golgi membrane protein GP73; golgi phosphoprotein 2; golgi protein, 73-kD; GOLPH2; GP73; HEL46; PSEC0257
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GOLPH2/GOLM1 using anti-GOLPH2/GOLM1 antibody (A02975-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH2/GOLM1 antigen affinity purified polyclonal antibody (Catalog # A02975-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH2/GOLM1 at approximately 73 kDa. The expected band size for GOLPH2/GOLM1 is at 73 kDa.
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