Western blot using GeneTex's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution.
Western blot using GeneTex's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution.
GRASP antibody
GTX48721
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetTamalin
Overview
- SupplierGeneTex
- Product NameGRASP antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1 microg. ELISA: 1:4000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.03 mg/ml
- ConjugateUnconjugated
- Gene ID56149
- Target nameTamalin
- Target descriptiontrafficking regulator and scaffold protein tamalin
- Target synonymsGrasp, general receptor for phosphoinositides 1-associated scaffold protein, GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein, GRP1-associated scaffold protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9JJA9
- Protein NameGeneral receptor for phosphoinositides 1-associated scaffold protein
- Scientific DescriptionTamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domain-containing protein that interacts with group 1 metabotropic glutamate receptors (mGluRs). The PDZ domain-containing amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs. The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins. It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161