
Western blot analysis of extracts from HUVEC cells, HepG2 cells and HeLa cells, using GRK5 antibody.
GRK5 Antibody
CSB-PA794868
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRK5
Overview
- SupplierCusabio
- Product NameGRK5 Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID2869
- Target nameGRK5
- Target descriptionG protein-coupled receptor kinase 5
- Target synonymsFP2025; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; GPRK5
- HostRabbit
- IsotypeIgG
- Protein IDP34947
- Protein NameG protein-coupled receptor kinase 5
- Scientific DescriptionSerine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Kunapuli P., Proc. Natl. Acad. Sci. U.S.A. 90:5588-5592(1993). The MGC Project Team, Genome Res. 14:2121-2127(2004).
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C
- UNSPSC12352203