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Bio-Connect Diagnostics

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tlrl-mtd | M-TriDAP | InvivoGen products available at Bio-Connect

M-TriDAP | tlrl-mtd | InvivoGen

M-TriDAP

tlrl-peklps | LPS-EK Ultrapure | InvivoGen products available at Bio-Connect

LPS-EK Ultrapure | tlrl-peklps | InvivoGen

LPS-EK Ultrapure

Pam3CSK4 induces a dose-dependent response in HEK-Blue™ hTLR2 cells. These cells were stimulated with increasing concentrations of Pam3CSK4. After overnight incubation, the NF-κB response was determined using HEK-Blue™ Detection, a SEAP detection medium, and by reading the optical density (OD) at 630 nm. 

Specific TLR2-mediated NF-κB activation by Pam3CSK4. THP1-Dual™ and THP1-Dual™ KO-TLR2 cells were stimulated with increasing concentrations of Pam3CSK4. After overnight incubation, the NF-κB response was determined using QUANTI-Blue™ Solution, a SEAP detection reagent, and by reading the optical density (OD) at 630 nm. The IRF response was assessed by measuring the activity of Lucia luciferase in the supernatant using QUANTI-Luc™. Data for the Lucia luciferase readout are shown as a fold increase over non-induced cells. As expected, THP1-Dual™ KO TLR2 cells did not respond to Pam3CSK4. Of note, due to TLR2 not directly signaling through an IRF-dependent pathway, Pam3CSK4  did not induce a significant fold-increase in the IRF-inducible reporter Lucia luciferase in the THP1-Dual™ cells.

tlrl-pms | Pam3CSK4 | InvivoGen products available at Bio-Connect

Pam3CSK4 | tlrl-pms | InvivoGen

Pam3CSK4

Pam3CSK4 (Pam3CysSerLys4) is a synthetic triacylated lipopeptide (LP) and a TLR2/TLR1 ligand. It is a potent activator of the pro-inflammatory transcription factor NF-κB [1, 2]. Pam3CSK4 mimics the acylated amino terminus of bacterial LPs.

Bacterial LPs are a family of pro-inflammatory cell wall components found in both Gram-positive and Gram-negative bacteria. The stimulatory activity of these LPs resides in their acylated amino terminus. These bacterial LPs are recognized by TLR2, a receptor that plays a pivotal role in detecting a diverse range of pathogen-associated molecular patterns (PAMPs) [3].

At the cell surface, TLR2 forms a heterodimer with co-receptors TLR1 or TLR6, depending upon either tri- or diacylation of the ligand. Once a ligand binds to either TLR2-TLR1 or TLR2-TLR6, a MyD88-dependent activation of NF-κB and AP-1 occurs, ultimately leading to an innate immune response. Recognition of Pam3CSK4, a triacylated LP, is mediated by TLR2 which cooperates with TLR1 through their cytoplasmic domain to induce the signaling cascade leading to the activation of NF-κB [4].

tlrl-sipgn | PGN-SAndi ultrapure, insoluble | InvivoGen products available at Bio-Connect

PGN-SAndi ultrapure, insoluble | tlrl-sipgn | InvivoGen

PGN-SAndi ultrapure, insoluble

tlrl-stfla | FLA-ST | InvivoGen products available at Bio-Connect

FLA-ST | tlrl-stfla | InvivoGen

FLA-ST

tlrl-zyn | Zymosan | InvivoGen products available at Bio-Connect

Zymosan | tlrl-zyn | InvivoGen

Zymosan

0215232301 | FIBROBLAST GROWTH FACTOR Basic | MP Biomedicals (Life Sciences) products available at Bio-Connect

FIBROBLAST GROWTH FAC... | 0215232301 | MP Biomedicals (Life Sciences)

FIBROBLAST GROWTH FACTOR Basic

0215670425 | SUDAN ORANGE G | MP Biomedicals (Life Sciences) products available at Bio-Connect

SUDAN ORANGE G | 0215670425 | MP Biomedicals (Life Sciences)

SUDAN ORANGE G

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