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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (bsm-33221M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

GRP78/Bip Monoclonal Antibody

BSM-33221M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetHSPA5
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GRP78/Bip Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12D9
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3309
  • Target name
    HSPA5
  • Target description
    heat shock protein family A (Hsp70) member 5
  • Target synonyms
    78 kDa glucose-regulated protein; binding-immunoglobulin protein; BIP; endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; GRP78; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 70 family protein 5; heat shock protein family A member 5; HEL-S-89n; HSP70 family protein 5; immunoglobulin heavy chain-binding protein
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP11021
  • Protein Name
    Endoplasmic reticulum chaperone BiP
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203