
The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
GRP94 antibody [2H3]
GTX50064
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetHSP90B1
Overview
- SupplierGeneTex
- Product NameGRP94 antibody [2H3]
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions:Immunofluorescence: Use at 1:100 ~ 1:200.Optimal working concentrations should be determined experimentally by the end user.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2H3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7184
- Target nameHSP90B1
- Target descriptionheat shock protein 90 beta family member 1
- Target synonymsECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1, endoplasmin, 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, epididymis luminal protein 35, epididymis secretory sperm binding protein Li 125m, heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, heat shock protein 90kDa beta family member 1, heat shock protein family C member 4, stress-inducible tumor rejection antigen gp96, tumor rejection antigen (gp96) 1, tumor rejection antigen 1
- HostMouse
- IsotypeIgG2a
- Protein IDP14625
- Protein NameEndoplasmin
- Scientific DescriptionThis gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5 exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22791/GTX22791_1116_IHC-P_w_23060620_550.webp)


![IHC-P analysis of human breast carcinoma tissue using GTX34773 GRP94 antibody [HSP90B1/1192].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34773/GTX34773_20200115_IHC-P_358_w_23060801_277.webp)

