Bio-Connect

GUSB / Beta Glucuronidase Antibody (Biotin)

LS-C185064
LifeSpan BioSciences
ApplicationsImmunoDiffusion, ImmunoElectrophoresis, Western Blot, ELISA
Product group Antibodies
TargetGUSB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GUSB / Beta Glucuronidase Antibody (Biotin)
  • Delivery Days Customer
    14
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ImmunoDiffusion, ImmunoElectrophoresis, Western Blot, ELISA
  • Applications Supplier
    ELISA, ID, IE, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID2990
  • Target name
    GUSB
  • Target description
    glucuronidase beta
  • Target synonyms
    beta-D-glucuronidase; beta-G1; beta-glucuronidase; BG; MPS7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of A431 cells using anti-GUSB antibody (A02234-1). Overlay histogram showing A431 cells stained with A02234-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GUSB Antibody (A02234-1,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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