Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (9D5) Monoclonal Antibody (bsm-51225M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (9D5) Monoclonal Antibody (bsm-51225M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
H2AFX (9D5) Monoclonal Antibody
BSM-51225M
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetH2AX
Overview
- SupplierBioss Antibodies
- Product NameH2AFX (9D5) Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), IHC-P(1:200-400)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9D5
- Concentration0.5 ug/ml
- ConjugateUnconjugated
- Gene ID3014
- Target nameH2AX
- Target descriptionH2A.X variant histone
- Target synonymsH2A histone family member X; H2A.X; H2A/X; H2AFX; H2AX histone; histone H2A.x; histone H2AX
- HostMouse
- IsotypeIgG1
- Protein IDP16104
- Protein NameHistone H2AX
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203