Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H2AK5ac Polyclonal Antibody (bs-53146R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H2AK5ac Polyclonal Antibody (bs-53146R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
H2AK5ac Polyclonal Antibody
BS-53146R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetH2AW
Overview
- SupplierBioss Antibodies
- Product NameH2AK5ac Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IF(ICC)(1:50-200), dot-blot(), ChIP-seq()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.95 ug/ul
- ConjugateUnconjugated
- Gene ID92815
- Target nameH2AW
- Target descriptionH2A.W histone
- Target synonymsHIST3H2A; histone 3, H2a; histone cluster 3 H2A; histone cluster 3, H2a; histone H2A type 3
- HostRabbit
- Protein IDQ7L7L0
- Protein NameHistone H2A type 3
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203