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ChIP assays were performed using HeLa cells, the Bioss antibody against H3K9/14ac (Cat. No. bs-53093R) and optimized primer pairs for qPCR. ChIP was performed with a HighCell# ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analyzed. IgG (5 μg/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. The figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.
ChIP assays were performed using HeLa cells, the Bioss antibody against H3K9/14ac (Cat. No. bs-53093R) and optimized primer pairs for qPCR. ChIP was performed with a HighCell# ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analyzed. IgG (5 μg/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. The figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.
ChIP assays were performed using HeLa cells, the Bioss antibody against H3K9/14ac (Cat. No. bs-53093R) and optimized primer pairs for qPCR. ChIP was performed with a HighCell# ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analyzed. IgG (5 μg/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. The figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.

H3K9/14ac Polyclonal Antibody

BS-53093R
Bioss Antibodies
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
DownloadDatasheet
Product group Antibodies
ReactivityFungus, Human, Mouse, Plant, Other Species
TargetH3C1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    H3K9/14ac Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Dot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IF(), dot-blot(), ChIP-seq()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.4 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8350
  • Target name
    H3C1
  • Target description
    H3 clustered histone 1
  • Target synonyms
    H3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
  • Host
    Rabbit
  • Protein IDP68431
  • Protein Name
    Histone H3.1
  • Reactivity
    Fungus, Human, Mouse, Plant, Other Species
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203