Anti-Ms CD154 Purified Low Endotoxin

EXBIO

Hamster Monoclonal to CD154 / CD40L (mouse)

7

The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Functional application: Blocking T cell costimulation in vivo. Flow cytometry: Recommended dilution: 4 microg/ml; positive control: 6-8 hour activated mouse splenocytes.

Functional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

MR-1

1 mg/ml

IgG1

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

Mouse

2°C to 8°C

12352203