
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Hec1 (phospho Ser 55) antibody
GTX70017
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetNDC80
Overview
- SupplierGeneTex
- Product NameHec1 (phospho Ser 55) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.26 mg/ml
- ConjugateUnconjugated
- Gene ID10403
- Target nameNDC80
- Target descriptionNDC80 kinetochore complex component
- Target synonymsHEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog, NDC80 homolog, kinetochore complex component, NDC80 kinetochore complex component homolog, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, kinetochore associated 2, kinetochore protein Hec1, kinetochore-associated protein 2, retinoblastoma-associated protein HEC
- HostRabbit
- IsotypeIgG
- Protein IDO14777
- Protein NameKinetochore protein NDC80 homolog
- Scientific DescriptionHEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161










