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Paraformaldehyde-fixed, paraffin embedded rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with HECA Polyclonal Antibody, Unconjugated (bs-9702R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with HECA Polyclonal Antibody, Unconjugated (bs-9702R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with HECA Polyclonal Antibody, Unconjugated (bs-9702R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

HECA Polyclonal Antibody

BS-9702R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHECA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HECA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51696
  • Target name
    HECA
  • Target description
    hdc homolog, cell cycle regulator
  • Target synonyms
    dJ225E12.1; HDC; HDCL; headcase homolog; headcase protein homolog; HHDC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBI9
  • Protein Name
    Headcase protein homolog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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