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Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10microg/ml.
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10microg/ml.
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10microg/ml.

Heme Oxygenase 1 antibody

GTX30748
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHmox1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Heme Oxygenase 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC: Use at an assay dependent dilution. WB: 1:2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID24451
  • Target name
    Hmox1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HEOXG, Heox, Hmox, Ho-1, Ho1, hsp32, heme oxygenase 1, heme oxygenase (decycling) 1, heme oxygenase (decyclizing) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06762
  • Protein Name
    Heme oxygenase 1
  • Scientific Description
    catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]
  • Reactivity
    Canine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Du D, Lv W, Su R, et al. Hydrolyzed camel whey protein alleviated heat stress-induced hepatocyte damage by activated Nrf2/HO-1 signaling pathway and inhibited NF-κB/NLRP3 axis. Cell Stress Chaperones. 2021,26(2):387-401. doi: 10.1007/s12192-020-01184-z
    Read this paper

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ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetHmox1
  • SizePrice
Figure 1. Western blot analysis of Heme Oxygenase 1/Hmox1 using anti-Heme Oxygenase 1/Hmox1 antibody (A00253-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/Hmox1 antigen affinity purified polyclonal antibody (Catalog # A00253-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/Hmox1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/Hmox1 is at 33 kDa.
Product group Antibodies
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Anti-Heme Oxygenase 1/Hmox1 Antibody Picoband(r)A00253-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetHmox1
  • SizePrice