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ProductsBack/Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Polyclonal Antibody

Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Polyclonal Antibody

CAU25859
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetHbegf
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Overview

  • Supplier
    Biomatik
  • Product Name
    Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID15200
  • Target name
    Hbegf
  • Target description
    heparin-binding EGF-like growth factor
  • Target synonyms
    AW047313; diphtheria toxin receptor; Dt; Dtr; Dts; Hegf; Hegfl; heparin binding epidermal growth factor-like growth factor; proheparin-binding EGF-like growth factor
  • Host
    Rabbit
  • Protein IDQ06186
  • Protein Name
    Proheparin-binding EGF-like growth factor
  • Scientific Description
    The Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hbegf using anti-Hbegf antibody (A01759-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hbegf antigen affinity purified polyclonal antibody (Catalog # A01759-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hbegf at approximately 20 kDa. The expected band size for Hbegf is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-Hbegf Antibody Picoband(r)A01759-4-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetHbegf
  • SizePrice