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ProductsBack/hHR23A (RAD23A) Goat Polyclonal Antibody
WB showing Anti-HR23A antibody detects endogenous human HR23A. Reactivity against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time.
WB showing Anti-HR23A antibody detects endogenous human HR23A. Reactivity against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time.
WB showing Anti-HR23A antibody detects endogenous human HR23A. Reactivity against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time.

hHR23A (RAD23A) Goat Polyclonal Antibody

TA319249
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetRAD23A
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    hHR23A (RAD23A) Goat Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5886
  • Target name
    RAD23A
  • Target description
    RAD23 homolog A, nucleotide excision repair protein
  • Target synonyms
    HHR23A; HR23A; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
  • Host
    Goat
  • Protein IDP54725
  • Protein Name
    UV excision repair protein RAD23 homolog A
  • Scientific Description
    Goat polyclonal anti-HR23A antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: RAD23A antibody at 3microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 34 kDa
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Figure 1. Western blot analysis of HHR23A/RAD23A using anti-HHR23A/RAD23A antibody (A03243-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHR23A/RAD23A antigen affinity purified polyclonal antibody (Catalog # A03243-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HHR23A/RAD23A at approximately 60 kDa. The expected band size for HHR23A/RAD23A is at 60 kDa.
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