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WB using anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining (right panel) using conventional anti-HIF-1alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.
WB using anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining (right panel) using conventional anti-HIF-1alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.
WB using anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining (right panel) using conventional anti-HIF-1alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.

HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

TA319212
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Human, Monkey, Mouse, Rat, Xenopus
TargetHIF1A
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3091
  • Target name
    HIF1A
  • Target description
    hypoxia inducible factor 1 subunit alpha
  • Target synonyms
    ARNT interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PAS domain-containing protein 8", "Class E basic helix-loop-helix protein 78", "Member of PAS protein 1; PASD8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16665
  • Protein Name
    Hypoxia-inducible factor 1-alpha
  • Scientific Description
    Rabbit polyclonal Hif-1 alpha hydroxy P564 antibody
  • Reactivity
    Bovine, Canine, Human, Monkey, Mouse, Rat, Xenopus
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203