HighFidelity AF594 PCR Labeling Kit, Preparation of AF594-labeled DNA probes by PCR

Jena Bioscience

HighFidelity AF594 PCR Labeling Kit, Preparation of AF594-labeled DNA probes by PCR

9

Other Application

Research Use Only

HighFidelity AF594 PCR Labeling Kit is designed to produce randomly AF594-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4kbp is feasible. dUTP-XX-AF594 is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50 % dUTP-XX-AF594 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-AF594/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 microl each (50% dUTP-XX-AF594 substitution, 100 microM dATP/dGTP/dCTP, 50 microM dTTP, 50 microM dUTP-XX-AF594).

-20°C

41116133