HighFidelity ORANGE PCR Labeling Testkit, Preparation of AF594-, ATTO594- and TexasRed-labeled DNA probes by PCR

Jena Bioscience

HighFidelity ORANGE PCR Labeling Testkit, Preparation of AF594-, ATTO594- and TexasRed-labeled DNA probes by PCR

5

Research Use Only

HighFidelity ORANGE PCR Labeling Testkit is designed to produce randomly AF594-, ATTO594- and TexasRed-modified DNA probes by PCR to find the optimal label for the orange emission wavelength range. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4kbp is feasible. All labeled-dUTP are efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50 % / 25 % labeled-dUTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of labeled-dUTP/dTTP ratio however, can easily be achieved with the single nucleotide format.

-20°C

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