HighYield T7 Cy3 RNA Labeling Kit, Preparation of randomly Cy3-modified RNA probes by in vitro transcription with UTP-X-CY3

Jena Bioscience

HighYield T7 Cy3 RNA Labeling Kit, Preparation of randomly Cy3-modified RNA probes by in vitro transcription with UTP-X-CY3

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Research Use Only

HighYield T7 Cy3 RNA Labeling Kit is designed to produce randomly Cy3-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY3 (also known as Cy3-17-UTP from GE Healthcare) is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-X-Cy3 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy3/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy3-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 microl each (35 % UTP-X-Cy3 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy3).

-20°C

41116133