Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5?g/mL)
Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5?g/mL)
His Tag Antibody (OAEA00010)
OAEA00010
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Other Application
Product group Antibodies
Overview
- SupplierAviva Systems Biology
- Product NameHis Tag Antibody (OAEA00010)
- Delivery Days Customer23
- Antibody SpecificityRecognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Other Application
- Applications SupplierWB (ECL): 1:1000~3000 (incubate for one hour at room temperature). IP: 1:50~200. IS: 1:500~2000. For best results with other assays (e.g.: DB, ELISA, etc), please determine optimal working dilution by titration test.
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHIS.H8
- Concentration1 mg/ml
- FormatLiquid 10mM PBS (pH 7.2) with 0.05% sodium azide
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionHis Tag Antibody
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203