Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (TA377049) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (TA377049) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody
TA377049
Overview
- SupplierOriGene
- Product NameHistone H2A.X (H2AFX) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID3014
- Target nameH2AX
- Target descriptionH2A.X variant histone
- Target synonymsH2A histone family member X; H2A.X; H2A/X; H2AFX; H2AX histone; histone H2A.x; histone H2AX
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-Histone H2AX-S139 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203