ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60346 Histone H2B antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60346 Histone H2B antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.
Histone H2B antibody - ChIP grade
GTX60346
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameHistone H2B antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2,000 - 1/10,000. ICC/IF: 1:1,000. ELISA: 1:1,000 - 1/5,000. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2.8 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203