The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
![ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX60874/GTX60874_20200909_ChIPassay_1_w_23061123_434.webp "ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg")
![ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Red : Primary antibody Green : Actin](https://www.genetex.com/upload/website/prouct_img/normal/GTX60874/GTX60874_20200909_ICCIF_165_w_23061123_111.webp "ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Red : Primary antibody Green : Actin")
![WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Dilution : 0.5microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60874/GTX60874_20200909_WB_270_w_23061123_552.webp "WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Dilution : 0.5microg/ml")
The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
Histone H3K14ac (Acetyl Lys14) antibody [RM130]
GTX60874
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameHistone H3K14ac (Acetyl Lys14) antibody [RM130] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (
- Application Supplier NoteWB: 0.5 microg/mL - 2 microg/mL. ICC/IF: 0.5 microg/mL - 2 microg/mL. ELISA: 0.2 microg/mL - 1 microg/mL. ChIP assay: 2 microg/mL - 10 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM130
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203