The GTX60886 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
![ChIP analysis of HeLa cells with or without Sodium Butyrate treatment using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX60886/GTX60886_20200909_ChIPassay_9_w_23061123_854.webp "ChIP analysis of HeLa cells with or without Sodium Butyrate treatment using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg")
![Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166] (2 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60886 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60886/GTX60886_20200909_ELISA_52_w_23061123_827.webp "Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166] (2 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60886 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.")
![ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Red : Primary antibody Green : Actin](https://www.genetex.com/upload/website/prouct_img/normal/GTX60886/GTX60886_20200909_ICCIF_166_w_23061123_884.webp "ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Red : Primary antibody Green : Actin")
![ICC/IF analysis of HepG2 cells using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60886/GTX60886_20200909_ICCIF_193_w_23061123_918.webp "ICC/IF analysis of HepG2 cells using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166].")
![WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Dilution : 0.5microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60886/GTX60886_20200909_WB_265_w_23061123_585.webp "WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Dilution : 0.5microg/ml")
The GTX60886 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Histone H3K18ac (Acetyl Lys18) antibody [RM166] - Orthogonal Validated
GTX60886
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetH3C1
Overview
- SupplierGeneTex
- Product NameHistone H3K18ac (Acetyl Lys18) antibody [RM166] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM166
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
- HostRabbit
- IsotypeIgG
- Modification TypeAcetylated
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203