ChIP analysis of sheared chromatin from 5x10? K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.