ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Figure 1A. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive TSH2B and MYT1 genes, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me3, H3K9me3 and H3K36me3 modifications and the unmodified H3K27 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K27me3 modification.
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Figure 1A. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive TSH2B and MYT1 genes, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me3, H3K9me3 and H3K36me3 modifications and the unmodified H3K27 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K27me3 modification.
Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade
GTX60816
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityAlgae, C. Elegans, Drosophila, Human, Mouse, Plant, Other Species
Overview
- SupplierGeneTex
- Product NameHistone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:200. Dot: 1:5,000. ELISA: 1:100 - 1:500. ChIP assay: 0.5-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityAlgae, C. Elegans, Drosophila, Human, Mouse, Plant, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203