The GTX60892 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.
![ICC/IF analysis of HepG2 cells using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60892/GTX60892_20200909_ICCIF_197_w_23061123_897.webp "ICC/IF analysis of HepG2 cells using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].")
![WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]. Dilution : 1microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60892/GTX60892_20200909_WB_324_w_23061123_600.webp "WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]. Dilution : 1microg/ml")
The GTX60892 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.
Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]
GTX60892
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetH3C1
Overview
- SupplierGeneTex
- Product NameHistone H3K27me3 (Tri-methyl Lys27) antibody [RM175]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM175
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
- HostRabbit
- IsotypeIgG
- Modification TypeMethylated
- ReactivityHuman, Mouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways. Tchasovnikarova IA et al., 2022 Jan 20, Mol CellRead more
- Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation. Kriz AJ et al., 2021 May 6, Mol CellRead more
- Xist Repeats A and B Account for Two Distinct Phases of X Inactivation Establishment. Colognori D et al., 2020 Jul 6, Dev CellRead more
- PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Wang CY et al., 2019 Jul 3, Nat CommunRead more
- Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Colognori D et al., 2019 Apr 4, Mol CellRead more
- SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X. Wang CY et al., 2018 Jul 12, CellRead more
- Repeat E anchors Xist RNA to the inactive X chromosomal compartment through CDKN1A-interacting protein (CIZ1). Sunwoo H et al., 2017 Oct 3, Proc Natl Acad Sci U S ARead more