ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 microl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and ALDOA and for the coding region of the myogenic differentiation gene (MYOD). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).