ChIP analysis of HeLa cells using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg
![ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Red : Primary antibody Green : Actin](https://www.genetex.com/upload/website/prouct_img/normal/GTX60878/GTX60878_20200909_ICCIF_175_w_23061123_537.webp "ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Red : Primary antibody Green : Actin")
![WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Dilution : 0.5microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX60878/GTX60878_20200909_WB_316_w_23061123_566.webp "WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Dilution : 0.5microg/ml")
![ELISA analysis of various methylated Histone H3 peptides using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. It specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1) and no cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60878/GTX60878_20230728_ELISA_23072801_454.webp "ELISA analysis of various methylated Histone H3 peptides using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. It specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1) and no cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.")
ChIP analysis of HeLa cells using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5microg
Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]
GTX60878
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetH3C1
Overview
- SupplierGeneTex
- Product NameHistone H3K4me1 (Mono-methyl Lys4) antibody [RM140]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3
- Application Supplier NoteWB: 0.2 microg/mL - 1 microg/mL. ICC/IF: 1 microg/mL - 2 microg/mL. ELISA: 0.2 microg/mL - 1 microg/mL. ChIP assay: 2 microg/mL - 10 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM140
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3 histone family, member A; H3/A; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3.1; histone H3/a
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203