ChIP analysis of sheared chromatin from 5x10? HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.